Long-term activity of biohybrid coatings of atrazine-degrading bacteria \u3ci\u3ePseudomonas\u3c/i\u3e sp. ADP

摘要

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol–gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about 94% during each assay cycle. Staining with CTC revealed that in spite of cultivation under non-growth conditions over a period of one year, immobilized cells were still vital and showed respiratory activity.